Businesses are seeking biodiversity indicators to help assess performance for a variety of business applications to address a range of internal and external drivers. However, challenges exist to develop indicators that can cut through the complexity of natural systems, display clear and simple measures of biodiversity, and be relevant to different business applications. Whilst some businesses are seeking one indicator to be used across a variety of business  applications, the reality is that assessing biodiversity performance for different business applications at different temporal and spatial scales will often require different indicators. This paper introduces a spectrum of business  applications for biodiversity indicators. The process introduced in this paper offers a contribution to the current dialogue on biodiversity indicators for business. It is designed to help businesses seeking to integrate biodiversity into  their business know where to start, by asking the right questions upfront and to seek out existing indicators that could help measure and track their biodiversity performance.